Brenneria nigrifluens Isolated from Aesculus hippocastanum L. Bark in Hungary

In Hungary, from the beginning of 19th century, horse-chestnut trees have been planted widely and are popular ornamental in public parks, along streets, gardens. summer 2015, longitudinal cracks on trunk branches intensive oozing brown liquid were observed a wound tree park Budapest. Some years later, 2018 2019, same symptoms found other locations Several bacteria reported that induce similar symptoms, including cankers bark trunks sticky, white, red, brown, or black oozing. These pathogens belong to genera Brenneria Lonsdalea. Bark exudate samples taken with aim identifying causal agent by conventional molecular methods. Our results confirmed isolated Aesculus hippocastanum genus phylogenetic analysis 16S rRNA gene region proved closest relation nigrifluens strains.